New paper: finding mutations in non-reference strains

Last year, we demonstrated that short-read sequencing is very useful to hunt down mutations causal for phenotypes in lines isolated from EMS mutant screens (Schneeberger et al., 2009).

However, this was for mutations induced in the Col-0 reference background, for which a very high-quality reference genome is available.

In a paper that just appeared online in Plant Physiology, we now show that we can reliably distinguish even rare new mutations from a sea of background SNPs in non-reference lines. Find the paper here.